20 Bp Ladder Sigma

Components sigma s pcr 20 bp low marker is provided in a solution of 10 mm tris hcl ph 8 0 with 1 0 mm edta.

20 bp ladder sigma. Search results for 50 bp at sigma aldrich. Directload pcr 100 bp low ladder ready to use marker for dna electrophoresis. Select up to 4 products. Please select more than one item to compare.

The biocompare ladders and markers search tool catalogs a wide variety of reagents for sizing nucleic acid and proteins in electrophoresis applications. It is a step ladder with 20 bp differences between the dna fragments and consists of purified and ligated blunt ended basic unit repeats of 20 bp. Pcr 20 bp low ladder has been used as a marker for dna gel electrophoresis. Pcr 20 bp low ladder for electrophoresis of pcr fragments.

The key to accurate band analysis is to use the correct dna ladder for your particular application. Sigma aldrich offers a variety of markers for nucleic acid size determination of sample separated by various gel electrophoresis methods. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel. By selecting the generuler dna ladder of interest in table 1 you will be directed to the corresponding product page for more details and ordering information generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of double stranded dna ranging from 10 bp.

For optimal resolution the recommended agarose gel concentration is 2 5. 100 bp dna ladder consists of 13 individual chromatography purified dna fragments and has reference bands at 2000 1500 and 600 bp for easy orientation 100 b. Thermo scientific o rangeruler 20 bp dna ladder 20 300 bp is designed for precise sizing of pcr products and other double stranded dna fragments in agarose or non denaturing polyacrylamide gels. The double stranded dna ladder can be visualized on 4 to 5 agarose gels for 10bp to 150bp fragments 2 to 3 agarose gels for 150bp to 330bp fragments 2 to 3 agarose 1000 gels or 6 to 10 polyacrylamide gels after ethidium bromide staining.

Ladders contain premixed fragments of purified dna rna or protein of known sizes in terms of base pairs or molecular weights. Components sigma s pcr 100 bp low marker is provided in a solution of 10 mm tris hcl ph 8 0 with 1 0 mm edta. Other notes contains 50 bands 20 1 000 bp in exact 20 bp repeats. Search results for 20 bp dna ladder at sigma aldrich.